Skip to main content
. Author manuscript; available in PMC: 2014 May 30.
Published in final edited form as: Cell Rep. 2013 Apr 25;3(5):1430–1439. doi: 10.1016/j.celrep.2013.03.042

Figure 3. XBP1 is deleted specifically in the adipocytes of the mammary gland.

Figure 3

(A) Xbp1 mRNA levels in fractionated mammary tissue from day 1 and day 10 lactation. Tissues from WT and XBP1ΔAd mice were separated into adipocyte and stromal-vascular (SV) fractions (n=4-5). (B) Laser Capture Microdissection (LCM) of mammary epithelial cells from mammary glands on day 1 lactation. Total RNA was extracted from the microdissected cells to determine levels of Xbp1 deletion. Pictures of histology illustrate the area of capture before and after dissection. (C) Average pup weights of litters nursed by WT and XBP1ΔMAC(LysM Cre) dams throughout lactation (n=5-6). (D) Average pup weights of litters nursed by WT and Adiponectin Cre XBP1ΔAd dams throughout lactation (n=5-6). (E) Mammary gland wet weights of WT and XBP1ΔAd dams (n=4-9). (F) Whole mount stains of mammary glands from WT and XBP1ΔAd mice at different stages of mammary development (non-pregnant (NonPG), day 1 or day 10 lactation). All error bars indicate +/- SEM. *denotes p<0.05 using standard t-test. See also Figure S3.