Fig. 2.

Podocytes express TLR3, RIG-I, and IPS-1. a Human podocytes were incubated with anti-TLR3-CTD mouse IgM Ab without (left panel, TLR3 Ab) or with (middle panel, TLR3 Ab + rTLR3-CTD) preincubation with recombinant hTLR3-CTD. Control cells were incubated with buffer (right panel, only 2nd Ab). All cultures were then stained with Alexa 488-anti-mouse IgM (TLR3 Ab) and DAPI. b The expression levels of RIG-I and IPS-1 proteins in murine podocytes (mPod) were analyzed by Western blot. Raw 264.7 (RAW), a murine macrophage cell line, was used as positive control. c RIG-I and IPS-1 expression levels in human podocytes (hPod) were tested by Western blot. HT1080 (HT), a human fibrosarcoma cell line, was used as positive control.