Table 1.
Primer sequences and product sizes
| Forward primer | Reverse primer | Product size, bp | |
|---|---|---|---|
| Mouse | |||
| TLR3 | ttgtcttctgcacgaacctg | cgcaacgcaaggattttatt | 206 |
| TRIF | cactgcctccagtctcttcc | gatcagtcagagggcccata | 213 |
| RIG-I | atctcaacaacggagccatc | gcggtcttagcatctccaac | 199 |
| Mda-5 | gcagtggctcaggagttacc | gcagtggctcaggagttacc | 244 |
| IPS-1 | ggacacactctggggactct | ggtcagggatgttgtgacct | 161 |
| TBK-1 | atggagttttgtccctgtgg | tgatgttgcctggcttgata | 169 |
| IRF3 | ctggctagagcatggaaacc | gatgccaaagtcagccatct | 170 |
| GAPDH | gaatgggaagcttgtcatcaa | ctaagcagttggtggtgcag | 288 |
| Human | |||
| TLR3 | acccataccaacatccctga | gccctcaaagtggatgagaa | 192 |
| TRIF | actgtgtcatccccttcctg | tgtcctgttccttcctccac | 186 |
| RIG-I | agagcacttgtggacgcttt | tgcaatgtcaatgccttcat | 213 |
| Mda-5 | catctgattggagctggaca | tgtgagcaaccaggacgtag | 250 |
| IPS-1 | ataagtccgagggcaccttt | gtgactaccagcacccctgt | 208 |
| TBK-1 | agcggcagagttaggtgaaa | tgagtgccttcttgatgtgc | 156 |
| IRF3 | gaggtgacagccttctaccg | tgcctcacgtagctcatcac | 176 |
| GAPDH | gtcagtggtggacctgacct | tgctgtagccaaattcgttg | 245 |