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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Alcohol Clin Exp Res. 2012 Jul 3;37(1):96–109. doi: 10.1111/j.1530-0277.2012.01850.x

Figure 2. PDCD4 expression was strongly induced in PCN in response to ETOH.

Figure 2

(A) PCNs were treated with ETOH 2.5mg/ml and 4mg/ml for 24 h. PDCD4 protein expression was determined by immunoblot analyses. Immunoblotting with anti-GAPDH was used as loading control. Lower panel depicts densitometric scanning analysis ratio of PDCD4 to GAPDH. Quantification data represents mean ± s.e.m, n=3. (B) PCNs were treated with 4mg/ml ETOH for different time points as indicated. Western analysis for PDCD4 and GAPDH was performed. Lower panel indicate the relative intensity of PDCD4 normalized to GAPDH (mean ± s.e.m, n=3). (C) 5 DIV PCNs were treated with ETOH (4mg/ml) for 24 h and the culture media containing ETOH were replaced with normal culture media for additional 12 h and 24 h. Equal amount of lysates were analyzed for PDCD4 and GAPDH protein expression. One way ANOVA was performed to establish statistical significance. In A, B * - P<0.05, when compared with untreated controls; ns-not significant compared with untreated control.