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FACS analysis of necrotic cell preperations. Jurkat cells in serum-free medium were induced to undergo necrosis by freeze-thaw (FT), heat, H2O2 ethanol (EtOH) or no treatment (controls). Following incubation at 37° for 30 min or 6 hr, cells were collected by centrifugation, stained with propidium iodide (PI) and (FITC )-labeled annexin V for analysis by flow cytometry. Annexin V binding is shown on the x-axis and propidium iodide binding is shown on the y-axis.
