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Kinetic analysis of DNA release from necrotic cells. Jurkat cells in serum-free medium were induced to undergo necrosis by freeze-thaw, heat, H2O2, ethanol or no treatment (controls), then incubated at 37° for 30 min, 2 h, 4 h and 6 h. Samples of culture supernatant were cleared of cells, then examined for DNA content in a PicoGreen® DNA assay. Data are representative of experiments performed at least 3 times. Error bar are present but are visible only for the freeze-thaw treatments.
