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. Author manuscript; available in PMC: 2013 Jul 26.
Published in final edited form as: Nat Immunol. 2010 Dec 26;12(2):160–166. doi: 10.1038/ni.1977

Figure 6.

Figure 6

PTBP2 promotes binding of AID to S region DNA. (a) Control or PTBP2 knock-down cells were stimulated with CIT for 48 h and chromatin immunoprecipitation (ChIP) was carried out with antibodies against AID, histone H3 or IgG control. Three-fold dilutions of ChIP DNA were analyzed by PCR for the presence of Sμ or Iμ (as control). (b) The amount of Sμ in anti-AID ChIP samples was quantified by real-time PCR. The graph depicts qPCR values expressed as relative ChIP units with scrambled assigned an arbitrary value of 100. ChIP units are derived from normalizing Ct values to input and then subtracting IgG ChIP Ct values as background. Results shown are the average of three independent experiments expressed with error bars representing standard deviation from the mean. p-value was determined by using the Student's t-test.

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