Table 2.
Percentage of splenic B cells that underwent CSR to IgG1 in individual experiments. CSR was measured by flow cytometry. Results from activated B cells expressing scramble shRNA, two PTBP2 shRNAs (PTBP2-1, PTBP2-2) and AID shRNA are shown.
Expt. | Scramble (%CSR to IgG1) | Scramble (%CSR to IgG1) | PTBP2-1 (% CSR to IgG1) | PTBP2-1 (% CSR to IgG1) |
---|---|---|---|---|
1 | 16.7 | 6.7 | 8.2 | 8 .2 |
2 | 16.5 | 1.0 | 1.3 | 1.1 |
3 | 18.3 | 7.9 | 4.9 | 11.6 |
4 | 20.3 | 6.0 | 6.8 | 5.1 |
5 | 21.5 | 12.3 | 14.3 | 16.6 |
6 | 18.9 | 2.9 | 3.7 | 4.5 |
7 | 11.2 | 3.9 | 3.0 | 3.7 |