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. 2012 Sep 6;9:32. doi: 10.1186/1476-9255-9-32

Figure 4.

Figure 4

Co-stimulatory molecule expression on pulmonary neutrophils in wild type and PAR-2-deficient mice following exposure to GC frass. BALB/c and PAR-2-deficient mice were administered a single intratracheal instillation of PBS or GC frass and whole lungs were isolated 20 h later. Cells were dissociated from the lungs, and stained for flow cytometry analysis. Neutrophil (gated on CD11c+, CD11b+, Gr1+) populations were analysed for CD86 (A + B), CD80 (C + D), and MHC class II (E + F). MFI is shown in the left panel and the frequency of parent is shown on the right panel. Values are means ± SEM, n = 8 mice per group and statistical analysis was performed by ANOVA (*p < 0.001).