Table 1.
Characterisation of 103 T. urticae ABC proteins
| Subfamily | Tetur ID 1 | Name | Length(AA) | Strand | Exons | Topology 2 | N-Glc3 | O-Glc4 |
|---|---|---|---|---|---|---|---|---|
| A (9) |
tetur01g00580 |
TuABCA-01 |
1659 |
- |
5 |
(6TM-NBD)2 |
5 |
|
| |
tetur01g15090 |
TuABCA-02 |
1639 |
+ |
4 |
(7/8TM-NBD)2 |
2 |
1 |
| |
tetur11g05030 |
TuABCA-03 |
1670 |
- |
5 |
(5/6TM-NBD)2 |
3 |
|
| |
tetur11g05040 |
TuABCA-04 |
1670 |
- |
5 |
(6TM-NBD)2 |
2 |
|
| |
tetur11g05200 |
TuABCA-05 |
1672 |
- |
5 |
(6TM-NBD)2 |
2 |
|
| |
tetur15g01990 |
TuABCA-06 |
1666 |
+ |
5 |
(6TM -NBD)2 |
3 |
|
| |
tetur25g01640 |
TuABCA-07 |
2302 |
+ |
20 |
(6TM-NBD)2 |
8 |
|
| |
tetur27g01890 |
TuABCA-08 |
2082 |
+ |
2 |
(7/4TM-NBD)2 |
5 |
27 |
| |
tetur30g01960 |
TuABCA-09 |
1651 |
- |
5 |
(6/8TM-NBD)2 |
3 |
|
| B (4) |
tetur11g04030 |
TuABCB-01 |
1294 |
+ |
15 |
(6TM-NBD)2 |
4 |
|
| |
tetur11g04040 |
TuABCB-02 |
1292 |
+ |
16 |
(6TM-NBD)2 |
4 |
|
| |
tetur17g02000 |
TuABCB-03 |
688 |
- |
8 |
4TM-NBD |
1 |
|
| |
tetur32g01330 |
TuABCB-04 |
656 |
- |
8 |
5TM-NBD |
3 |
|
| C (39) |
tetur01g07880 |
TuABCC-01 |
1507 |
+ |
11 |
5TM-(5TM-NBD)2 |
1 |
2 |
| |
tetur01g10390 |
TuABCC-02 |
1529 |
+ |
11 |
7TM-(5TM-NBD)2 |
|
|
| |
tetur01g15310 |
TuABCC-03 |
1299 |
+ |
15 |
(5/6TM-NBD)2 |
5 |
1 |
| |
tetur01g15330 |
TuABCC-04 |
1328 |
+ |
14 |
(7TM-NBD)2 |
5 |
|
| |
tetur01g15340 |
TuABCC-05 |
1338 |
+ |
14 |
(8/7TM-NBD)2 |
3 |
|
| |
tetur03g02240 |
TuABCC-06 |
1375 |
- |
12 |
(6TM-NBD)2 |
2 |
|
| |
tetur03g07460 |
TuABCC-07 |
1481 |
- |
11 |
6TM-(5TM-NBD)2 |
2 |
|
| |
tetur03g07490 |
TuABCC-08 |
1483 |
- |
11 |
6TM-(5TM-NBD)2 |
1 |
|
| |
tetur03g07840 |
TuABCC-09 |
1457 |
- |
5 |
5TM-(6TM-NBD)2 |
1 |
|
| |
tetur03g09800 |
TuABCC-10 |
1490 |
+ |
11 |
6TM-(5TM-NBD)2 |
|
1 |
| |
tetur03g09880 |
TuABCC-11 |
1495 |
+ |
11 |
4TM-(5TM-NBD)2 |
1 |
1 |
| |
tetur04g04360 |
TuABCC-12 |
1522 |
+ |
5 |
8TM-(5TM-NBD)2 |
3 |
|
| |
tetur04g05540 |
TuABCC-13 |
1503 |
+ |
8 |
6TM-(5TM-NBD)2 |
5 |
|
| |
tetur04g07860 |
TuABCC-14 |
1501 |
- |
8 |
7TM-(4TM-NBD)2 |
3 |
|
| |
tetur04g07910 |
TuABCC-15 |
1493 |
- |
8 |
6TM-(5TM-NBD)2 |
2 |
|
| |
tetur05g01110 |
TuABCC-16 |
1522 |
- |
11 |
4TM-(5TM-NBD)2 |
6 |
1 |
| |
tetur05g04300 |
TuABCC-17 |
1490 |
+ |
8 |
5TM-(6TM-NBD)2 |
6 |
2 |
| |
tetur06g00360 |
TuABCC-18 |
1516 |
+ |
11 |
6TM-(5TM-NBD)2 |
4 |
1 |
| |
tetur06g03510 |
TuABCC-19 |
1343 |
+ |
6 |
(6TM-NBD)2 |
2 |
|
| |
tetur06g03560 |
TuABCC-20 |
1339 |
+ |
6 |
(5/7TM-NBD)2 |
2 |
|
| |
tetur07g04290 |
TuABCC-21 |
1324 |
- |
5 |
(6TM-NBD)2 |
1 |
|
| |
tetur07g04410 |
TuABCC-22 |
1324 |
+ |
5 |
(6TM-NBD)2 |
1 |
|
| |
tetur09g00580 |
TuABCC-23 |
1506 |
+ |
8 |
7TM-(6TM-NBD)2 |
2 |
|
| |
tetur09g00590 |
TuABCC-24 |
1496 |
+ |
9 |
6TM-(6TM-NBD)2 |
1 |
|
| |
tetur09g04610 |
TuABCC-25 |
1521 |
+ |
8 |
7TM-(6TM-NBD)2 |
4 |
1 |
| |
tetur09g04620 |
TuABCC-26 |
1501 |
+ |
8 |
6TM-(5TM-NBD)2 |
5 |
|
| |
tetur11g02060 |
TuABCC-27 |
1499 |
- |
11 |
4TM-(6TM-NBD)2 |
1 |
|
| |
tetur11g02120 |
TuABCC-28 |
1492 |
+ |
11 |
6TM-(4TM-NBD)2 |
2 |
1 |
| |
tetur11g05990 |
TuABCC-29 |
1683 |
- |
15 |
5TM-(6TM-NBD)2 |
6 |
|
| |
tetur14g02290 |
TuABCC-30 |
1309 |
- |
16 |
(5/7TM-NBD)2 |
4 |
|
| |
tetur14g02300 |
TuABCC-31 |
1312 |
- |
16 |
(5/7TM-NBD)2 |
5 |
|
| |
tetur14g02310 |
TuABCC-32 |
1308 |
- |
16 |
(6/7TM-NBD)2 |
3 |
1 |
| |
tetur14g02320 |
TuABCC-33 |
1309 |
- |
16 |
(7TM-NBD)2 |
2 |
1 |
| |
tetur14g02330 |
TuABCC-34 |
1309 |
- |
16 |
(5/7TM-NBD)2 |
2 |
|
| |
tetur16g03480 |
TuABCC-35 |
1513 |
- |
11 |
6TM-(5TM-NBD)2 |
2 |
1 |
| |
tetur23g02452 |
TuABCC-36 |
1344 |
- |
6 |
(8/6TM-NBD)2 |
2 |
|
| |
tetur25g01780 |
TuABCC-37 |
1525 |
+ |
12 |
5TM-(5TM-NBD)2 |
|
|
| |
tetur28g01950 |
TuABCC-38 |
1287 |
+ |
7 |
(6TM-NBD)2 |
3 |
|
| |
tetur40g00010 |
TuABCC-39 |
1490 |
+ |
11 |
5TM-(5TM-NBD)2 |
|
1 |
| D (2) |
tetur05g06640 |
TuABCD-01 |
829 |
+ |
4 |
5TM-NBD |
2 |
1 |
| |
tetur35g01360 |
TuABCD-02 |
656 |
+ |
5 |
5TM-NBD |
4 |
|
| E (1) |
tetur30g01400 |
TuABCE-01 |
614 |
- |
2 |
NBD-NBD |
|
|
| F (3) |
tetur20g02610 |
TuABCF-01 |
612 |
+ |
4 |
NBD-NBD |
3 |
|
| |
tetur29g00620 |
TuABCF-02 |
584 |
+ |
1 |
NBD-NBD |
3 |
|
| |
tetur32g00490 |
TuABCF-03 |
718 |
- |
4 |
NBD-NBD |
4 |
|
| G (23) |
tetur01g16280 |
TuABCG-01 |
915 |
- |
6 |
NBD-7TM |
1 |
1 |
| |
tetur01g16290 |
TuABCG-02 |
643 |
+ |
3 |
NBD-6TM |
1 |
|
| |
tetur02g11270 |
TuABCG-03 |
689 |
+ |
11 |
NBD-6TM |
1 |
|
| |
tetur02g11400 |
TuABCG-04 |
811 |
- |
2 |
NBD-6TM |
3 |
1 |
| |
tetur02g13710 |
TuABCG-05 |
810 |
- |
2 |
NBD-6TM |
3 |
|
| |
tetur03g04350 |
TuABCG-06 |
687 |
- |
2 |
NBD-6TM |
2 |
|
| |
tetur04g04550 |
TuABCG-07 |
720 |
+ |
2 |
NBD-6TM |
3 |
1 |
| |
tetur05g05440 |
TuABCG-08 |
721 |
+ |
2 |
NBD-8TM |
1 |
1 |
| |
tetur06g05430 |
TuABCG-09 |
646 |
- |
12 |
NBD-7TM |
3 |
|
| |
tetur09g01930 |
TuABCG-10 |
685 |
+ |
9 |
NBD-5TM |
2 |
|
| |
tetur09g01950 |
TuABCG-11 |
682 |
+ |
9 |
NBD-5TM |
1 |
|
| |
tetur09g01960 |
TuABCG-12 |
683 |
+ |
9 |
NBD-5TM |
3 |
|
| |
tetur09g01970 |
TuABCG-13 |
687 |
+ |
9 |
NBD-6TM |
3 |
|
| |
tetur09g01980 |
TuABCG-14 |
680 |
+ |
9 |
NBD-6TM |
4 |
|
| |
tetur09g01990 |
TuABCG-15 |
687 |
+ |
9 |
NBD-6TM |
2 |
4 |
| |
tetur09g02000 |
TuABCG-16 |
682 |
+ |
9 |
NBD-7TM |
3 |
|
| |
tetur11g00520 |
TuABCG-17 |
792 |
- |
1 |
NBD-8TM |
2 |
3 |
| |
tetur11g01800 |
TuABCG-18 |
790 |
- |
1 |
NBD-8TM |
2 |
3 |
| |
tetur17g02510 |
TuABCG-19 |
832 |
- |
4 |
NBD-4TM |
2 |
1 |
| |
tetur17g03970 |
TuABCG-20 |
700 |
- |
2 |
NBD-8TM |
5 |
|
| |
tetur19g01160 |
TuABCG-21 |
775 |
- |
1 |
NBD-7TM |
4 |
2 |
| |
tetur33g01719 |
TuABCG-22 |
760 |
+ |
1 |
NBD-6TM |
1 |
|
| |
tetur37g01090 |
TuABCG-23 |
779 |
- |
1 |
NBD-7TM |
1 |
14 |
| H (22) |
tetur01g03530 |
TuABCH-01 |
827 |
+ |
1 |
NBD-8TM |
6 |
7 |
| |
tetur01g05940 |
TuABCH-02 |
766 |
- |
6 |
NBD-6TM |
2 |
2 |
| |
tetur01g05970 |
TuABCH-03 |
734 |
- |
6 |
NBD-6TM |
1 |
1 |
| |
tetur03g03080 |
TuABCH-04 |
767 |
- |
2 |
NBD-8TM |
3 |
|
| |
tetur03g05300 |
TuABCH-05 |
738 |
- |
1 |
NBD-6TM |
1 |
|
| |
tetur04g06390 |
TuABCH-06 |
707 |
+ |
1 |
NBD-7TM |
2 |
1 |
| |
tetur05g05000 |
TuABCH-07 |
713 |
+ |
1 |
NBD-7TM |
3 |
|
| |
tetur07g05200 |
TuABCH-08 |
764 |
- |
4 |
NBD-6TM |
|
|
| |
tetur12g03340 |
TuABCH-09 |
744 |
- |
1 |
NBD-5TM |
1 |
|
| |
tetur12g03910 |
TuABCH-10 |
713 |
+ |
1 |
NBD-7TM |
3 |
|
| |
tetur13g02010 |
TuABCH-11 |
703 |
- |
1 |
NBD-8TM |
2 |
1 |
| |
tetur13g02060 |
TuABCH-12 |
723 |
- |
1 |
NBD-7TM |
3 |
|
| |
tetur18g00230 |
TuABCH-13 |
735 |
- |
2 |
NBD-8TM |
4 |
20 |
| |
tetur19g01780 |
TuABCH-14 |
692 |
- |
1 |
NBD-7TM |
5 |
2 |
| |
tetur21g00940 |
TuABCH-15 |
716 |
+ |
2 |
NBD-6TM |
1 |
1 |
| |
tetur26g02620 |
TuABCH-16 |
708 |
- |
10 |
NBD-7TM |
2 |
4 |
| |
tetur28g00780 |
TuABCH-17 |
726 |
- |
1 |
NBD-8TM |
1 |
2 |
| |
tetur28g00870 |
TuABCH-18 |
720 |
+ |
1 |
NBD-7TM |
3 |
1 |
| |
tetur30g00890 |
TuABCH-19 |
721 |
- |
1 |
NBD-7TM |
2 |
|
| |
tetur32g01710 |
TuABCH-20 |
804 |
- |
1 |
NBD-6TM |
2 |
4 |
| |
tetur36g00240 |
TuABCH-21 |
777 |
- |
1 |
NBD-6TM |
3 |
1 |
| tetur36g00630 | TuABCH-22 | 704 | - | 1 | NBD-6TM | 1 |
1T. urticae gene models can be accessed at the ORCAE genome portal, http://bioinformatics.psb.ugent.be/orcae/overview/Tetur[120].
2 transmembrane helices (TMs) were predicted using the SCAMPI server [128] while nucleotide binding domains (NBD) were determined using the ScanProSite facility [121] and Prosite profile PS50893; ( )2 indicates that the T. urticae ABC protein is a full transporter.
3 N-glycosylation sites were predicted using NetNGlyc 1.0 server [131]; only N-glycosylation site with a “potential” score > 0.5 and with a jury agreement were taken into account.
4O-glycosylation sites were predicted using NetOGlyc 3.1 server [132]; if the G-score was higher than 0.5 the residue was considered to be O-glycosylated; in this table the total number of O-glycosylated sites (glycosylated serines and threonines) is shown.