Table 3.
conc. (mg/mL) |
Digestion area (mm
2
)* |
||
---|---|---|---|
WT | K698N | K698Q | |
0.05 |
125.7 ± 4.2 |
126.7 ± 8.5 |
71.1 ± 10.6 |
0.1 |
194.9 ± 8.8 |
151.8 ± 9.3 |
100.8 ± 20.1 |
0.2 |
217.9 ± 16.6 |
189.9 ± 1.7 |
138.0 ± 13.2 |
0.4 |
279.1 ± 2.1 |
233.8 ± 9.6 |
182.9 ± 18.6 |
0.8 | 308.1 ± 22.0 | 268.8 ± 4.1 | 203.7 ± 10.7 |
*Area at each data point was calculated (A = πr2) by measuring the radius ® of the digestion ring.