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Degradation of GS2 and RCA within isolated, intact chloroplasts. A) Chloroplasts were isolated from wild type Arabidopsis leaves and incubated in the dark for the indicated number of hours. Immediately after incubation, intact chloroplasts were purified on a Percoll gradient, and only intact chloroplasts were used for immunoblot analysis with the indicated antibodies. Pixels were quantified for GS2 (B) and the two RCA isoforms (C).
