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. 2001 May 1;29(9):1898–1905. doi: 10.1093/nar/29.9.1898

Figure 4.

Figure 4

PAGE gel image of the ethidium bromide-stained and UV-visualised 98 nt PCR fragment derived from pUC19 and the C7-amino-modified 7-deaza-dATP analogues 9–11. Lane 1, molecular weight markers; lane 2, a PCR containing all four natural triphosphates, dATP, dCTP, dGTP and dTTP; lane 3, a PCR containing dTTP, dCTP and dGTP does not result in the formation of any product; lane 4, a PCR containing dTTP, dCTP, dGTP and 9; lane 5, a PCR containing dTTP, dCTP, dGTP and 10; lane 6, a PCR dTTP, dCTP, dGTP and 11; lane 7, molecular weight markers. All triphosphates 9–11 (Fig. 3) are substrates for Taq polymerase during PCR.