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. 2001 May 1;29(9):1898–1905. doi: 10.1093/nar/29.9.1898

Figure 5.

Figure 5

PAGE gel image of the ethidium bromide-stained and UV-visualised 62 nt PCR fragment derived from pUC19 and the C7-amino-modified 7-deaza-dATP analogues 9–11 and dUTP imidazole conjugates 12 and 13. Lane 1, molecular weight markers; lane 2, a PCR containing all four natural triphosphates, dATP, dCTP, dGTP and dTTP; lane 3, a PCR containing dCTP and dGTP does not result in the formation of any product; lane 4, a PCR containing dTTP, dCTP, dGTP and 9; lane 5, a PCR containing dATP, dCTP, dGTP and 12; lane 6, a PCR dCTP, dGTP, 9 and 12; lane 7, a PCR dCTP, dGTP, 9 and 13; lane 8, molecular weight markers. Full-length 62 nt PCR products were formed using 9 and 12, and 9 and 13 in place of dATP and dUTP, respectively, using Taq polymerase.