Skip to main content
. 2001 May 1;29(9):1898–1905. doi: 10.1093/nar/29.9.1898

Figure 6.

Figure 6

PAGE gel image of the ethidium bromide-stained and UV-visualised PCR fragments derived from pUC19 and the C7-amino-modified 7-deaza-dATP analogues 9–11 (Fig. 4) following incubation with XbaI. Lane 1, molecular weight markers; lane 2, control 98 nt PCR product containing all four natural triphosphates, dATP, dCTP, dGTP and dTTP in the absence of XbaI; lane 3, the 98 nt PCR product containing all four natural triphosphates, dATP, dCTP, dGTP and dTTP following incubation with XbaI; lane 4, the 98 nt PCR product containing dTTP, dCTP, dGTP and 9 following incubation with XbaI; lane 5, the 98 nt PCR product containing dTTP, dCTP, dGTP and 10 following incubation with XbaI; lane 6, the 98 nt PCR product containing dTTP, dCTP, dGTP and 11 following incubation with XbaI; lane 7, molecular weight markers. Incorporation of analogues 9–11 into recognition site for XbaI (T^CTAGA) results in inhibition of cleavage.