Table 1. Summary of substrate properties of the nucleotide analogues.
| Triphosphate analogue | Length of product | |
| |
62 nt |
98 nt |
|
9 (alkyne amino
C7-dATP) |
+++ |
+++ |
|
10 (Z-alkene
amino C7-dATP) |
no. exp |
++ |
|
11 (alkane amino
C7-dATP) |
no. exp |
++ |
|
9 and 12 |
+ |
– |
| 9 and 13 | ++ | + |
In each case, the indicated nucleotide was used as a replacement for dATP of dATP and dTTP during PCR. Results are shown for synthesis of PCR products of lengths of 62–98 nt. Details of conditions are described in Materials and Methods. The absence of PCR product is indicated by –. ′+′ indicates that full-length PCR product was obtained (the number of ′+′ signs is proportional to the efficiency/yield of PCR product). ‘No exp’ indicates that no PCR experiment was made.