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. Author manuscript; available in PMC: 2013 Jul 26.
Published in final edited form as: Nat Protoc. 2012 Feb 2;7(2):374–393. doi: 10.1038/nprot.2011.446

Figure 3.

Figure 3

Retroviral and targeting constructs. Schematic representation of miR30-based retroviral (LMP, TGMP) and Col1a1-targeting (cTGM, cTtGM and cTtRM) vectors used in this protocol. Constructs are shown as they appear after genomic integration. TGMP is cloned within a self-inactivating (SIN) retroviral backbone and when copied into the genome the 5′ LTR promoter activity is disrupted—represented by blunted arrows. The three Col1a1-targeting constructs shown differ only in the inducible promoter (TRE or TREtight) and fluorescent spacer before shRNAmir (GFP or turboRFP).