Skip to main content
. Author manuscript; available in PMC: 2013 Jul 26.
Published in final edited form as: Nat Protoc. 2012 Feb 2;7(2):374–393. doi: 10.1038/nprot.2011.446
Component Amount per tube Final
EcoRI buffer, 10× 5 µl 1×
BSA (10 mg ml−1), 100× 0.5 µl 0.1 mg ml−1
Plasmid DNA 10 µg (miR30 plasmid)/5 µg (cTGM)
EcoRI (20,000 U ml−1) 1.5 µl 30 U
XhoI (20,000 U ml−1) 1.5 µl 30 U
DNase-free H2O Up to 50 µl