| Component | Amount per tube (µl) | Final |
|---|---|---|
| pCAGs-FlpE (2 mg ml−1 in H2O) | 12.5 | 25 µg |
| cTGM (targeting vector in H2O) | 25 | 50 µg |
| KH2 ES cells (in PBS) | 900 | 4.5 × 106 cells |
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