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. Author manuscript; available in PMC: 2013 Jul 26.
Published in final edited form as: Nat Protoc. 2012 Feb 2;7(2):374–393. doi: 10.1038/nprot.2011.446
Component Amount per tube (µl) Final
NEB buffer 1, 10× 5 1×
BSA (10 mg ml−1), 100× 0.5 0.1 mg ml−1
cTGM (1 mg ml−1) 10 10 µg
NcoI (10,000 U ml−1) 1.0 10 U
AgeI (5,000 U ml−1) 2.0 10 U
DNase-free H2O 31.5