| Component | Amount per well (µl) | Final |
|---|---|---|
| DNase-free H2O | 16.5 | |
| PCR buffer, 10× | 2.5 | 1× |
| dNTPs (2.5 mM each) | 2.5 | 0.25 mM |
| Forward (shRNA-specific) primer (10 µM) | 1 | 0.4 µM |
| Reverse (common) primer (10 µM) | 1 | 0.4 µM |
| Taq polymerase | 0.5 |
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