| PCR reaction | ||
|---|---|---|
| Component | Amount per well (µl) | Final |
| H2O | 33.5 | |
| PCR buffer, 10× | 5 | 1× |
| MgSO4 (50 mM) | 1 | 1 mM |
| DNTP (2.5 mM each) | 5 | 0.25 mM each |
| 5′miR-Xho (10 µM) | 2 | 0.4 µM |
| 3′miR-Eco (10 µM) | 2 | 0.4 µM |
| Pfx platinum DNA polymerase | 0.5 | 1.25 U |
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