| Restriction digest | ||
|---|---|---|
| Component | Amount per well (µl) | Final |
| Purified PCR product | 30 | |
| EcoRI buffer, 10× | 5 | 1× |
| BSA (10 mg ml−1) 100× | 0.5 | 0.1 mg ml−1 |
| XhoI (20,000 U ml−1) | 1 | 20 U |
| EcoRI (20,000 U ml−1) | 1 | 20 U |
| DNase-free H2O | 12.5 | |
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