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. Author manuscript; available in PMC: 2013 Jul 26.
Published in final edited form as: Nat Methods. 2009 Oct 18;6(11):805–808. doi: 10.1038/nmeth.1393

Figure 3. In vitro and in vivo differentiation of iPSCs generated with 3 compound treatment.

Figure 3

(a) Micrographs show embryoid bodies (EB) generated from iPSCs and in vitro differentiation into ectodermal (βIII tubulin), mesodermal (brachyury) and endodermal (PDX1) cell types. Scale bars, EB: 100 µm; others 10 µm (b) RT-PCR showing expression of representative lineage markers and the absence of OCT4 mRNA expression in differentiating cells. U- undifferentiated, D- differentiated. (c) Teratomas generated in nude mice from iPSCs (3 independent colonies tested) consist of tissues from all three germ layers. Left panel: 1- muscle, 2- neural epithelium; middle panel: 1- skin, 2- gut epithelium; right panel: 1- bone, 2- cartilage. Scale bars, 20 µm.