Table 2.
CO stripes and tracers injected |
Case number | Diameter (mm) | Interinjection distance (μm)a | Label overlap (%)b |
N single-labeled cells |
N double-labeled cells | Percentage double-labeled cells |
Figures | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Pale medial (Tracer 1) | Pale lateral (Tracer 2) | Tracer 1 | Tracer 2 | Percentage of total labeled cellsc | Percentage of cells labeled with Tracer 1d | Percentage of cells labeled with Tracer 2e | |||||||
CTBgmdf | CTB-488l | 326 | 0.39 × 0.61 | 0.25 × 0.53 | 466 | 88.6 | 1215 | 4195 | 51 | 0.93 | 4.03 | 1.22 | 5, 6A–E |
CTB 647l | CTB-488l | 326 | 0.22 × 0.65 | 0.25 × 0.53 | 1139 | 84.9 | 1376 | 1055 | 32 | 1.28 | 2.27 | 2.94 | 5, 6F–J |
CTBg | CTB-488 | 325 | 0.32 × 0.33 | 0.34 × 0.73 | 1262 | 92.9 | 946 | 1110 | 69 | 3.05 | 6.80 | 5.85 |
aDistance between the lateral edge of medial-most injection site and medial edge of lateral-most injection site, measured across CO stripes.
bArea of overlap of the two labels/smallest area of single-labeled field × 100.
cCalculated as follows: N double-labeled cells * 100/(N tracer 1-labeled cells + N tracer 2-labeled cells + N double-labeled cells).
dCalculated as follows: N double-labeled cells * 100/(N cells labeled with Tracer 1 + N double-labeled cells).
eCalculated as follows: N double-labeled cells * 100/(N cells labeled with Tracer 2 + N double-labeled cells).
fThis injection straddled a thick stripe medially.