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. 2013 Jul 26;8(7):e69985. doi: 10.1371/journal.pone.0069985

Figure 3. EMSA for nucleic acid binding.

Figure 3

Binding of mIHF-80 to (a) circular and (b) linear DNA was analyzed by running the reaction mixture on agarose gel and visualizing by EtBr staining. mIHF-80 binds DNA non-specifically in a concentration-dependent manner. (a) In the reaction mix, 18, 36, 75, 150, 300, 450, 600 or 900, (lanes 2–9) were incubated with circular supercoiled DNA at 37°C for 60 min. (b) To monitor binding to linear DNA, a similar reaction was carried out with 18, 37, 75, 150, 300, 450, 600 or 900 ng mIHF-80 (lanes 2–9) to monitor gel shift. Lane 1 is control with no protein in both gels.