FIGURE 1.

ET activates a Notch gene reporter and induces Notch target genes in mouse macrophages. A, as described under “Experimental Procedures,” RAW 264.7 cells containing a Notch luciferase gene reporter were created. Luciferase levels were then determined in the Notch reporter cell line (RAW-Notch) after a 7-h exposure to 10 nm ET (10 nm EF plus 10 nm PA) or 1 mm 6MB-cAMP. The values in the bar graph represent normalized average relative luciferase units (RLU) from three independent experiments. B–E, BMDMs were cultured as described under “Experimental Procedures.” These cells were exposed for 6 h to 10 nm ET (10 nm EF and 10 nm PA), and RT-qPCR was used to measure levels of Hes1, Hey1, Il2ra, and Il7r. Bar graphs are representative of three independent experiments. For the BMDMs, each experiment used a different culture generated from a different mouse. Error bars indicate mean ± S.D. *, p < 0.05.