FIGURE 4.

Expression level of Notch signaling components in human monocytes after exposure to ET or 6MB-cAMP. A–C, human monocytes were isolated and exposed for 6 h to 10 nm ET (10 nm EF and 10 nm PA) or 1 mm 6MB-cAMP. RT-qPCR was then used to quantify transcript levels in three independent experiments. Each experiment used monocytes obtained from a different donor. D, human monocytes were exposed to 10 nm ET (10 nm EF and 10 nm PA) for 6 h, and immunoblot analyses were performed with antibodies recognizing RBP-J and antibodies against GSK-3β (loading control). E, the bar graph represents the densitometry analysis of three RBP-J immunoblots. Error bars indicate mean ± S.D. *, p < 0.05; **, indicates p < 0.005.