FIGURE 2.

Cdc123 is important for integrity of the eIF2 complex. A and C–E, integrity of the eIF2 complex in cdc123 mutants was analyzed by immunoprecipitation. Levels of eIF2 subunit proteins in WCE and immunoprecipitates (IP) were determined by Western analysis. A, Gcd11-myc was precipitated from a diploid cdc123Δ327 mutant and control strains. B, synthetic lethal interaction of GCD11-myc and cdc123Δ327 alleles. Strains of the indicated genotypes were crossed and the meiotic progeny was analyzed by tetrad dissection. The allele segregation pattern is symbolized by vertical and horizontal lines. C, flag-Gcd11 was precipitated from a haploid cdc123Δ327 mutant and control strains. D and E, Sui2 and Sui3 were precipitated, and associated Gcd11 was analyzed in a cdc123Δ327 mutant strain (D) and in a pGALL-cdc123–1 strain grown in galactose medium at 25 °C (permissive) or shifted to glucose medium at 37 °C for 4 h (restrictive) (E). The asterisk indicates IgG heavy chain.