FIGURE 2.
Hey2 inactivation inhibits osteoblast function in vitro. Osteoblast-enriched cells were harvested from calvariae of male or female Oc-Cre+/−;Hey2Δ/Δ mice (Hey2 null, black bars), or littermate Hey2loxP/loxP controls of the same sex (Control, white bars) and cultured under conditions favoring osteoblastogenesis. Total RNA was extracted, and mRNA was reverse-transcribed and amplified by qRT-PCR in the presence of specific primers. Data are expressed as Alpl copy number, corrected for Rpl38 copy number. Values are means ± S.E., n = 3–4. Alkaline phosphatase activity was determined in cells extracted with Triton X-100, and data are expressed as nanomoles of p-nitrophenol/min/μg of total protein. Values are means ± S.E., n = 6. +, significantly different between day 7 and day 0, p < 0.05. *, significantly different between HEY2 and control, p < 0.05.