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. 2013 Jun 13;288(30):21618–21629. doi: 10.1074/jbc.M113.481077

FIGURE 4.

FIGURE 4.

Knockdown of Acsl4 did not change cellular total acyl-CoA levels except with the addition of exogenous AA where Acsl4 knockdown reduced arachidonoyl-CoA levels. A, effect of Acsl4 knockdown in INS 832/13 cells on total CoA species after a 4-h incubation with either vehicle or 50 μm oleate (OA) or 50 μm AA (final concentration). B, long chain acyl-CoA species from vehicle control. C, long chain acyl-CoA species from 50 μm oleate treated cells. D, long chain acyl-CoA species from 50 μm arachidonate-treated cells. 16:1-CoA, palmitoleoyl-CoA; 16:0-CoA, palmitoyl-CoA; 18:3-CoA, linolenoyl-CoA; 18:2-CoA, linoleoyl-CoA; 18:1-CoA, oleoyl-CoA; 18:0-CoA, stearoyl-CoA; 20:4-CoA, arachidonoyl-CoA; 20:1-CoA, eicosanoyl-CoA; 20-CoA, arachidoyl-CoA. The values are reported as means ± S.E. from three separate experiments carried out in triplicate. *, p < 0.05 relative to siControl.