FIGURE 3.

Amph activates LGC-55 channels. Representative composite currents recorded in LGC-55 injected oocytes perfused with control solution (A), 0.1 mm (B), and 1 Mm (C) Amph. Currents were stimulated by voltage steps from −160 to + 100 mV in 20-mV increments. D, representative LGC-55 mediated currents generated by Amph and cocaine at −60 mV membrane potential. E, dose-response curve of Amph-induced currents (n = 12). Data points recorded at −60 mV were normalized to the maximal values and fitted to a Michaelis-Menten equation. F, representative current-voltage curve generated by 1 mm Amph after subtraction of basal currents (0 mm Amph) in the presence of 96 (solid line) and 8 (dashed line) mm external chloride.