FIGURE 10.
Coupling effects of RCL and strand 3C substitutions on α1PDX reactivity with furin. A, double mutation cycle for substitution of serpin B8 RCL and strand 3C sequences into α1PDX. Lower case letters indicate the relative change in ka resulting from the mutations indicated by the arrow. B, fold-changes in ka for reactions of α1PDX with furin and the indicated furin loop variants resulting from individual or combined RCL and s3C substitutions in α1PDX. The coupling index indicates the ratio of the relative changes in ka for one set of mutations in the presence or absence of the second set of mutations. A value of 1 indicates no coupling.