TABLE 5.
Furin-PC chimeras | Serpin B8 |
α1PDX |
α1PDX-serpin B8 P6–P5′ |
α1PDX-YE |
α1PDX-serpin B8 P6–P5′-YE |
|||||
---|---|---|---|---|---|---|---|---|---|---|
ka | SI | ka | SI | ka | SI | ka | SI | ka | SI | |
m−1 s−1 | m−1 s−1 | m−1 s−1 | m−1 s−1 | m−1 s−1 | ||||||
Wild-type | 2.3 ± 0.2 × 105 | 2.2 ± 0.1 | 1.1 ± 0.1 × 106 | 1.1 ± 0.1 | 1.1 ± 0.1 × 105 | 1.4 ± 0.1 | 2.1 ± 0.7 × 105 | 1.9 ± 0.1 | 1.5 ± 0.2 × 103 | 1.3 ± 0.2 |
F-PC1 | 9.8 ± 1.2 × 104 | 1.4 ± 0.1 | 2.6 ± 0.5 × 106 | 1.7 ± 0.2 | 7.1 ± 1.4 × 104 | 1.3 ± 0.1 | 9.3 ± 1.7 × 105 | 1.3 ± 0.1 | 5.6 ± 0.1 × 103 | NDa |
F-PC2 | 6.4 ± 1.4 × 104 | 1.6 ± 0.2 | 1.1 ± 0.2 × 106 | 1.5 ± 0.1 | 5.3 ± 0.5 × 104 | 0.9 ± 0.1 | 1.7 ± 0.2 × 105 | 1.2 ± 0.1 | 9.2 ± 0.7 × 102 | ND |
F-PC4 | 1.3 ± 0.1 × 105 | 1.6 ± 0.1 | 1.0 ± 0.2 × 106 | 1.4 ± 0.2 | 6.4 ± 0.9 × 104 | 1.6 ± 0.1 | 2.4 ± 0.5 × 105 | 1.3 ± 0.2 | 3.4 ± 0.2 × 103 | ND |
F-PC5/6 | 4.9 ± 0.6 × 104 | 1.1 ± 0.1 | 2.2 ± 0.4 × 106 | 1.5 ± 0.2 | 6.4 ± 1.5 × 104 | 0.9 ± 0.1 | 9.4 ± 1.6 × 105 | 1.2 ± 0.2 | 2.6 ± 0.3 × 104 | ND |
F-PACE4 | 9.4 ± 0.9 × 104 | 1.8 ± 0.1 | 1.6 ± 0.3 × 106 | 1.9 ± 0.3 | 1.3 ± 0.1 × 105 | 0.8 ± 0.2 | 2.4 ± 0.5 × 105 | 1.4 ± 0.3 | 1.0 ± 0.1 × 103 | ND |
F-PC7 | 2.5 ± 0.4 × 105 | 2.1 ± 0.1 | 1.0 ± 0.2 × 106 | 1.4 ± 0.1 | 6.0 ± 0.7 × 104 | 1.3 ± 0.1 | 2.4 ± 0.2 × 105 | 1.1 ± 0.0 | 3.0 ± 0.1 × 103 | ND |
298–300 AAA | 6.1 ± 1.3 × 104 | 1.5 ± 0.3 | 1.8 ± 0.1 × 106 | 1.2 ± 0.1 | 1.9 ± 0.8 × 104 | 1.0 ± 0.1 | 1.3 ± 0.1 × 105 | 0.8 ± 0.2 | 1.5 ± 0.1 × 103 | ND |
a ND, not determined.