FIGURE 7.

TWEAK-induced activation of SGEF and stimulation of migration require TRAF2 function. A, U87 and U118 glioma cells were transfected with one of two independent siRNA oligonucleotides targeting TRAF2 (TRAF2-1 and TRAF2-2) or with siRNA targeting non-mammalian luciferase (Ctrl). After 72 h, cells were lysed, and lysates were immunoblotted (WB) as indicated. B, U87 glioma cells were transiently transfected with siRNA targeting non-mammalian luciferase (Ctrl) or TRAF2 (TRAF2-1) for 48 h. Cells were then serum-reduced (0.5% FBS) for an additional 16 h and treated with TWEAK for 2 min. SGEF activation in cell lysates was assessed using RhoG G15A-GST constructs. C, U87 and U118 cells were transiently transfected with siRNA targeting non-mammalian luciferase (Ctrl) or two independent siRNA oligonucleotides targeting TRAF2 (TRAF2-1 and TRAF2-2). After 24 h, cells were plated on glass slides precoated with 10 μg/ml laminin and cultured an additional 16 h in reduced-serum (0.5% FBS) medium. Cells were either left untreated or treated with TWEAK, and glioma cell migration was assessed over 24 h. Data represent the average of 10 replicates. *, p < 0.01.