FIGURE 3.
Sedimentation coefficient distributions, c(s), obtained from the sedimentation profiles for free Fasm and CaM and the Fasm-CaM complex at variable molar ratios. Molecular mass of the CaM-Fasm complex (∼42 kDa) is consistent with of 2:1 binding mode. Loading concentrations were at 60 and 20 μm for CaM and Fasm, respectively. Three CaM-Fasm complexes have been prepared at 0.5:1, 1:1, and 2:1 CaM:Fasm stoichiometries with Fasm concentration set constant at 20 μm. Rotor speed was set at 40,000 rpm (20 °C), and scans were acquired at 280 nm.