FIGURE 6.

Binding of FVIII to LDLR fragments in the presence of ScFv iKM33. A, in SPR, immobilized LDLR cluster was tested for binding with FVIII (full size recombinant FVIII, 275 nm) in the absence of iKM33 (1) or in its presence at 275 nm (2), 550 nm (3), or 1375 nm (4). Curve 5 corresponds to injection of buffer. RU, resonance units. B, in a similar way, the immobilized CR doublets 2-3, 3-4, and 4-5 were tested for binding with FVIII (275 nm) in the absence of iKM33 (light gray bars) or in its presence at 275 nm (dark gray bars) or 1375 nm (black bars). The resulting signals were recorded after 3 min of the association and are expressed in percentages of those obtained in the absence of iKM33.