FIGURE 7.

Binding of RAP and FVIII to the mutants of CR.4-5 and LDLR cluster. In SPR, CR.4-5 (A and C), CR.4-5 W144S/W193S (B and D), LDLR cluster (E and G), and its triple-mutant W66S/W144S/W193S (F and H) were immobilized and tested for binding with RAP (0.6, 1.3, 2.5, and 5 nm) (A, B, E, and F) and FVIII (full size recombinant FVIII, 50, 100, 150, and 200 nm) (C, D, G, and H). Injections of the buffer only were used as controls. For each pair of a particular LDLR fragment and its mutant tested versus a given ligand, the signals are shown in the same scale. CR domains affected by mutagenesis are marked by *. RU, resonance units.