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SDS-12.5% PAGE. Precipitation (P) (lanes 1, 3, 5, and 7) and supernatant (S) (lanes 2, 4, 6, and 8) of cell extract of E. coli containing melB expression plasmid. Lane M, marker; lanes 1 and 2, wild type; lanes 3 and 4, V359A; lanes 5 and 6, H332A; lanes 7 and 8, F513A. Arrows indicates the bands of melB pro-tyrosinase.
