TABLE 1.

Oligonucleotides used for site-directed mutagenesis

Name	Sequence (5′ →3′)a	Length	Use
MelBH332A-f1	GCGaactgggtgggaggattc	21	Forward primer for H332A
MelBH332A-r1	gacgttgttgtgaatagcttctaagttcatc	31	Reverse primer for H332A
MelBV359A-f1	ccagtggccgcgtatgatccgattttc	27	Forward primer for V359A
MelBV359A-r1	TGcactgctcatatggccagcaccccag	28	Reverse primer for V359A
MelBF513A-f1	agcggcagccttgaggacagtaactg	26	Forward primer for F513A
MelBF513A-r1	AGCgttgaagacactgcccacaaaattctg	30	Reverse primer for F513A
MelBC522A-f1	gacaagtgcgctcagcaagagcaag	25	Forward primer for C522A
MelBC522A-r1	TGCgttactgtcctcaaggctgccgctgaag	31	Reverse primer for C522A
MelBC525A-f1	gctcagcaagagcaagagggtgttttg	27	Forward primer for C525A
MelBC525A-r1	TGCcttgtcacagttactgtcctcaaggctg	31	Reverse primer for C525A
MelBC522A/C525A-f1	aagGCAgctcagcaagagcaag	22	Forward primer for C522A/C525A
MelBC522A/C525A-r1	gtcTGCgttactgtcctcaaggctgccgctgaag	34	Reverse primer for C522A/C525A

^a The changed nucleotides are shown in capitals, and the codons corresponding to the amino acid residues to be changed are underlined.