Figure 1. Expression and solubility of PST_Mh constructs.

The PST constructs were expressed in E. coli AD202 followed by cell disruption and centrifugation. The soluble (Sup) and insoluble (Pel) fractions were analyzed by SDS-PAGE and Coomassie Blue staining. Bands corresponding to the predicted sizes of the protein variants were observed (indicated with an asterisk). The increased intensity of the bands of the MalE-fusions indicated increased expression compared to the non-fusion protein. Furthermore, the truncated protein (MalE-Δ20PST_Mh) variant demonstrated the best solubility.