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. 2013 Jul 26;8(7):e69888. doi: 10.1371/journal.pone.0069888

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© 2013 Lindhout et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Capillary electrophoresis was used to analyze the reactions of GD3-FCHASE and CMP-Neu5Ac catalyzed by the three bacterial PSTs. Shown here are the reactions stopped at approximately 15% conversion. The large peak at ∼9.4 min represents the starting material and subsequent peaks are the products with additional Neu5Ac residues added. Acceptor plus 2 and 10 Neu5Ac products are indicated with arrows. The chain length of the products generated by PST_Nm (18 Neu5Ac) and PST_Mh (16 Neu5Ac) were significantly longer than that of PST_Ec (7 Neu5Ac). The electropherograms of the reactions have been overlaid on the same axis to aid in the comparison.