Table 2. Biochemical Properties of Bacterial Polysialyltransferases1.
| Specific activity2 | CMP-Neu5Ac Kinetics2 | ||||
| Enzyme | GD3-FCHASE (mU/mg) | GT3-FCHASE (mU/mg) | Km (mM) | Vmax (µmol/min/mg) | Vmax/Km |
| PSTMh | 1433.4±83.8 | 2670.8±92.1 | 1.1±0.05 | 1519.0±96.2 | 1380.9 |
| PSTNm | 939.6±222.2 | 1885.7±173.1 | 5.7±0.7 | 1318.5±40.3 | 251.9 |
| PSTEc | 322.1±56.1 | 225.5±50.3 | 4.9±0.7 | 800.4±112.4 | 162.4 |
1
Experiments were performed in triplicate (n = 3) with mean values and standard error reported.
2
Experiments were performed with enzymes from three separate purification batches in duplicate (n = 6) with mean values and standard error reported.