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Effect of Cf-GP on MMP and TIMP-1 protein expression in AGS cells. (A and B) Cells were treated with 0, 5, 10 or 20 μg/ml Cf-GP for 24 h. Western blot analysis and RT-PCR were performed as described. (C) AGS cells were cultured in six-well plates and treated with Cf-GP (0, 5, 10 or 20 μg/ml) for 24 h and analyzed by gelatin zymography. Cf-GP, Capsosiphon fulvescens glycoprotein; MMP, matrix metalloproteinase; TIMP, tissue inhibitors of metalloproteinases.
