Table 2. Effect of EB on RNase III–R1.1 RNA complex stability.
|
–EB |
+EB (40 µM) |
Kd (nM) | 14.4 ± 6.9 | 20.7 ± 0.9 |
Gel shift assays used 5′-32P-labeled R1.1 RNA (see Materials and Methods). The reported values are the averages of two experiments, along with the maximum error.