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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Exp Eye Res. 2013 Mar 23;114:120–127. doi: 10.1016/j.exer.2013.03.008

Figure 2.

Figure 2

Hyaluronic acid in control (upper) and recovering (lower) chick choroids. Hyaluronic acid is visualized using biotinylated-HABP (HA-binding region of aggrecan protein) and fluorescein-streptavidin. Significant HA accumulation (indicated by green labeling) is detected in the choroidal stroma of treated eyes between blood vessels (BV) and lymphatic channels (asterisks) following four days of recovery and throughout the treatment period. A, D, F, H) contralateral control choroids. B, E, G, I) recovering choroids. C; Negative control in which the biotinylated-HABP was omitted Blue staining represents the 4,6-diamidino-2-phenylindole (DAPI) staining of the nuclei. Scale bar= 50 μm. From: Summers Rada, et al., Increased hyaluronan synthase-2 mRNA transcripts and hyaluronan accumulation accompany choroidal thickening during the recovery from induced myopia. Invest. Ophthalmol. Vis. Sci. 51;6172–6179. 2010. Reproduced with permission © Association for Research in Vision and Ophthalmology.