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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nanomedicine. 2013 Feb 27;9(7):912–922. doi: 10.1016/j.nano.2013.02.006

Figure 1. Synthesis and PEGylation of cationic shell-crosslinked knedel-like (cSCK) nanoparticles.

Figure 1

The starting material was a block copolymer, consisting of a hydrophobic styrene block and a hydrophilic primary amine-bearing block (PAEA-b-PS). A PEG-NHS ester (1.5 kDa) was used to generate poly(acrylamidoethylamine-graft-polyethyleneglycol)-block-polystyrene (PAEA-g-PEG-b-PS). The process of micellization, followed by crosslinking with an activated diester of PEGylated polymers is identical to that of the non-PEGylated polymers. cSCK particles were conjugated with Alexa Fluor 488. Representative transmission electron photomicrographs show uniform size and shape of the non-PEG cSCK (parent; 100% primary amine (pa); cSCK-pa100) and the particle modified with 5 of the 1.5 kDa PEG on each block-polystyrene, termed cSCK-5PEG (cSCK- pa100-5PEG). Bar=50 nm.