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. 2013 Jul 26;8(7):e71019. doi: 10.1371/journal.pone.0071019

Figure 2. Analytical sensitivity of triplex and reference method PCRs on samples from experimentally infected animals.

Figure 2

(A) Viral RNA, extracted from blood samples taken at various time points post challenge from 2 animals (circles or squares) experimentally infected with CSFV and analysed by triplex RT-PCR (filled symbols) or CSFV RT-nPCR-TaqMan assay (open symbols). (B) Viral RNA, extracted from homogenised spleen samples taken from 6 pigs euthanized at various time points post challenge with ASFV and analysed by triplex RT-PCR (filled symbols) and ASFV-PCR (open symbols).