Table 1. Viruses used to determine analytical specificity of the triplex RT-PCR assay.
Virus | Subgenotype/genotype | Isolate |
CSFV | 1.1 | Alfort 187 |
1.2 | Brescia | |
1.3 | Guatemala HC#4009 | |
2.1 | UK2000/7.1 | |
2.2 | SP399/96 | |
2.3 | Rostock | |
3.1 | Congenital Tremor | |
3.3 | CBR/93 | |
3.4 | Kanagawa | |
ASFV | I | Ang72*, Ba71V*, Ben 97/1, BF07*, CV97*, Dakar 59, E70*, E75*, Haiti*, HOLLAND 86, Lis 60, ORI 84, ORI 85, Ss88*, |
II | Grg 2007/8 | |
III | ||
IV | ||
V | Moz 64*, Moz 79, TEN 89/1 | |
VI | ||
VIII | Lil 90/1, MwLil20/1*, DOWA, MAN 89/2 | |
IX | Ken06.B1*, Ken07.Eld1*, Uga 95/1, Ug03H* | |
X | Bur 84/1, BUR 90/2 | |
XVII | ZIM 92/1 | |
XXI | RSA/1/96 | |
Not determined | CAM 1/87 P4, CAM 89/7, CON 2006/1, DEIGHTON, KAN B 89/2, KEF 89/2, KEF 89/9, MALTA MSF2A, MGR 1/2005, MVMT 90/1, NUR 86, SIY 92/1, UGA 95/2 | |
BVDV I | 1a | C24V |
BVDV II | 2a | 502643 |
BDV | 1 | S137/4 |
Samples obtained from Instituto Nacional de Investigacion in Madrid, Spain.
All other ASFV samples were obtained from The Pirbright Institute, UK.
All CSFV strains were obtained from AHVLA reference collection.