Table 2. Primer and probes used in the triplex RT-PCR.
Target | Primer/Probe | Sequence 5′–3′ |
CSFV | CSF100-F | ATG CCC AYA GTA GGA CTA GCA |
CSF-Probe 1 | FAM-TGG CGA GCT CCC TGG GTG GTC TAA GT-BHQ1 | |
CSF192-R | CTA CTG ACG ACT GTC CTG TAC | |
ASFV | ASFVp72IVI_L | GAT GAT GAT TAC CTT YGC TTT GAA |
ASFV-CY5 | Cy5-CCA CGG GAG GAA TAC CAA CCC AGT G-DDQ II | |
ASFVp72IVI_R | TCT CTT GCT CTR GAT ACR TTA ATA TGA | |
IC | EGFP1-F | GAC CAC TAC CAG CAG AAC AC |
EGFP1-HEX | HEX-AGC ACC CAG TCC GCC CTG AGC A-BHQ1 | |
EGFP2-R | GAA CTC CAG CAG GAC CAT G |